Comparative Antioxidant Capacity of Seed and Fruit Ethanol Extracts of Bitter Melon (Momordica charantia L.) Using FRAP Assay

Luysi Ariani; Faradiba Abdul Rasyid; Dewi Yuliana; Rahmawati Rahmawati; Andi Muflihunna; Rezki Amriati Syarif

doi:10.52365/jecp.v6i1.1628

Comparative Antioxidant Capacity of Seed and Fruit Ethanol Extracts of Bitter Melon (Momordica charantia L.) Using FRAP Assay

Luysi Ariani, Faradiba Abdul Rasyid, Dewi Yuliana, Rahmawati Rahmawati, Andi Muflihunna, Rezki Amriati Syarif

Abstract

Momordica charantia L. (bitter melon) is traditionally consumed as both food and medicine in Indonesia. Its fruit and seeds contain bioactive compounds such as saponins, flavonoids, polyphenols, and unsaturated fatty acids with recognized antioxidant potential. This study compared the antioxidant activity of ethanol extracts from bitter melon seeds and fruit using the Ferric Reducing Antioxidant Power (FRAP) assay. Extraction was conducted by ultrasound-assisted extraction (UAE) with ethanol as solvent, and antioxidant activity was expressed as quercetin equivalents (mg QE/g extract). The seed extract showed an antioxidant value of 8.680 mg QE/g, while the fruit extract exhibited 8.626 mg QE/g. The statistical analysis showed that the p-value was greater than 0.05, indicating no significant difference between the two extracts. This suggests that both extracts exhibit comparable antioxidant activity. These results highlight that both seeds and fruit of M. charantia may serve as promising natural antioxidant sources for functional food and pharmaceutical applications.

Keywords

Momordica charantia; bitter melon; seeds; fruit; FRAP; antioxidant

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References

Aminah, Aminah, A. Muflihunna, and Zainal Abidin. 2016. “Uji Aktivitas Antioksidan Fraksi Etil Asetat Daun Wungu (Graptophyllum pictum (Linn) Griff) dengan Metode FRAP (Ferric Reducing Antioxidant Power).” Jurnal Ilmiah As-Syifaa, 8(1): 39–44. https://doi.org/10.33096/jifa.v8i1.156.

Hussain, Ashiq, Haya Fatima, Madiha Komal, Samina Kauser, Shazia Yaqub, Saima Akram, Faiza Iftikhar Gorsi, Ayesha Najam, Aqsa Atta, Sidrah and Abdeen Elsiddig Elkhedir. 2024. “Evaluation of Peel, Flesh and Seeds of Bitter Gourd (Momordica charantia L.) for Biologically Active Components, Through Development of Powders and Ethanolic Extracts.” Discover Applied Sciences, 6: 432. https://doi.org/10.1007/s42452-024-06086-8.

Hasanah, Nur, and Dede Rival Novian. 2020. “Analisis Ekstrak Etanol Buah Labu Kuning (Cucurbita moschata D.).” Jurnal Poltekkes Tegal 9(1): 54–59. https://doi.org/10.30591/pjif.v9i1.1758.

Munadi, Rachmin. 2020 “Analisis Komponen Kimia dan Uji Rimpang Jahe Merah (Zingiber officinale Rosc. Var rubrum),” Cokroaminoto Journal of Chemical Science 2(1): 1–6.

Ratnasari, Baiq Desi, Diah Miftahul Aini, Imam Syahputra Yamin, and Gladeva Yugi Antari. 2022. “Antiradical Activity Study of Momordica charantia L. Seeds Based on DPPH and its Secondary Metabolites Analysis,” Jurnal Ilmiah Medicamento 8(1): 56–62. https://doi.org/10.36733/medicamento.v8i1.3352.

Sriwijayanti, Apriningtias, Boima Situmeang, Nani Yulianti, Dian Susvira, and Holisha Widiyanto. 2024. “Phytochemical Screening and Antioxidant Activity from Methanol Extract of Bitter Pare Seeds (Momordica charantia L.).” Jurnal Beta Kimia 4(1): 77–84. https://doi.org/10.35508/jbk.v4i1.15487.

Tambunan, Pravil Mistryanto, Syarifah Nadia, and Fatimah Az Zahra Siregar. 2024. “Skrining dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight) Walp) dengan Metode FRAP (Ferric Reducing Antioxidant Power).” Forte Journal 4(1): 66–72. https://doi.org/10.51771/fj.v4i1.702.

Wardani, Gusti Agung Ayu Kusuma, Ketut Agus Adrianta, Ni Nyoman Wahyu Udayani, Ni Nyoman Yudianti Mendra, Ni Luh Gede Erica Fridayana, and Ni Made Dharma Shantini Suena. 2025. “Aktivitas Antioksidan Ekstrak Etanol Kulit Biji dan Buah Bakau (Xylocarpus granatum J.Koenig) dengan Metode DPPH dan FRAP,” Jurnal Ilmiah Medicamento 11(1): 86–98. https://doi.org/10.36733/medicamento.v11i1.9426.

Yuda Kusuma, Ikhwan, and Yuli Maesaroh. 2020. “Aktivitas Buah Pare (Momordica charantia L.) sbagai Herbal Anti Hiperglikemia pada Kondisi Diabetes Melitus: Literature Review.” Jurnal Farmasi indonesia 12(2): 186–193. https://doi.org/10.35617/jfionline.v12i2.20.

DOI: http://dx.doi.org/10.52365/jecp.v6i1.1628