Effectiveness of Ethanol Extract Dosage of Arabian Bidara Leaf (Zizphus spina-christi (L.) Desf.) on Reduction of Hypercholesterolemia in Mus musculus

Valenata Valenata; Gaby Ayu Patricia; Khoirul Ngibad

doi:10.52365/jecp.v6i1.1449

Effectiveness of Ethanol Extract Dosage of Arabian Bidara Leaf (Zizphus spina-christi (L.) Desf.) on Reduction of Hypercholesterolemia in Mus musculus

Valenata Valenata, Gaby Ayu Patricia, Khoirul Ngibad

Abstract

Hypercholesterolemia is one of the main risk factors for cardiovascular disease, which is characterized by increased cholesterol levels in the blood. The leaves of Ziziphus spina-christi (Arabian bidara) are known to contain flavonoids, saponins, tannins, and alkaloids that have the potential to provide antihypercholesterolemia effects. This study aimed to evaluate the effectiveness of ethanol extract of Z. spina-christi leaves on the reduction of total cholesterol in hypercholesterolemia mice. Extraction is carried out by the maceration method using 96% ethanol and produces a yield of 3.8%. A total of 36 male mice were divided into six groups (n=6): negative control, positive control (propylthiouracil induction), drug control (simvastatin), and three extract treatment groups with doses of 14, 28, and 56 mg/20 g BB. The treatment was given orally for four days, then cholesterol levels were analyzed using the enzymatic method CHOD-PAP. The results showed that the positive control cholesterol levels increased significantly (189.3 ± 6.2 mg/dL) compared to the negative control (112.7 ± 10.3 mg/dL). The administration of the extract lowered cholesterol levels gradually: 170.4 ± 6.3 mg/dL (10%), 159.6 ± 7.6 mg/dL (15.7%), and 147.3 ± 11.7 mg/dL (22.2%). Simvastatin decreased more (117.7 ± 12.5 mg/dL; 37.8%). It was concluded that bidara arabic leaf extract has antihypercholesterolemia potential with a multifactorial mechanism, although its effectiveness is lower than that of simvastatin.

Keywords

arabic bidara leaves; ethanol extract; hypercholesterolemia; mice

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References

Abdulrahman, Mahmoud Dogara, Ali Muhammad Zakariya, Harmand A. Hama, Saber W. Hamad, Sawsan S. Al-Rawi, Sarwan W. Bradosty, and Ahmad H. Ibrahim. 2022. Ethnopharmacology, Biological Evaluation, and Chemical Composition of Ziziphus spina-christi (L.) Desf.: A Review. Advances in Pharmacological and Pharmaceutical Sciences 2022: 1–36. https://doi.org/10.1155/2022/4495688.

Ayunda, Rahmah Dara, and Soufa Malita. 2024. “Utilization of Flavonoid Compounds as Antioxidants in Hypercholesterolemia: A Literature Study.” Unram Medical Journal 13(3): 177–187. https://doi.org/10.29303/jk.v13i3.5388.

Bobaya, Selfiano J., Vina Z. Latuconsina, and Nathalie Kailola. 2023. “The Effect of Giving Moringa Plant Leaf Extract (Moringa Oleifera) on Blood Sugar Levels in Mice.” Molucca Medica 16(1): 88–97. https://doi.org/10.30598/molmed.2023.v16.i1.88.

Cao, Shixi, Mengqi Liu, Yao Han, Shouren Li, Xiaoyan Zhu, Defeng Li, Yinghua Shi, and Boshuai Liu. 2024. “Effects of Saponins on Lipid Metabolism: The Gut–Liver Axis Plays a Key Role.” Nutrients 16(10): 1514. https://doi.org/10.3390/nu16101514.

Chaerunnisa, Rosmita, and Supardi U.S. 2021. “Percentage Decrease in Lead Heavy Metal Content in Green Shellfish (Perna viridis) After the Depuration Process by Jakarta Bay Fishermen.” EduBiologia: Biological Science and Education Journal 1(2): 121. https://doi.org/10.30998/edubiologia.v1i2.9573.

Ciumărnean, Lorena, Mircea Vasile Milaciu, Octavia Runcan, Stefan Cristian Vesa, Andreea Liana Rachis, Vasile Negrean, Mirela-Georgiana Perné, Valer Ioan Donca, Teodora-Gabriela Alexescu, Ioana Para, and Gabriela Dogaru. 2020. “The Effects of Flavonoids in Cardiovascular Diseases.” Molecules 25(18): 4320. https://doi.org/10.3390/molecules25184320.

Cosme, Fernanda, Alfredo Aires, Teresa Pinto, Ivo Oliveira, Alice Vilela, and Berta Gonçalves. 2025. “A Comprehensive Review of Bioactive Tannins in Foods and Beverages: Functional Properties, Health Benefits, and Sensory Qualities.” Molecules 30(4): 800. https://doi.org/10.3390/molecules30040800.

Hamka, Zulfahmi, and Nurul Ilmi Muis. 2025. “Effect of Different Emulsifier Concentrations on Bidara Leaf Extract Lotion Preparation (Ziziphus spina-christi L.) On Physical Quality.” Jurnal Kesehatan Yamasi Makassar 9(2): 66–77. https://doi.org/10.59060/jurkes.v9i2.382.

Hastiana, Yetti, Sapta Handaiyani, and Icke Agustin. 2022. “Test of Phytocemical Levels of Bidara (Ziziphus spina-christi L.) Potential as Medicinal Plants.” Mangifera Edu 6(2): 182-96. https://doi.org/10.31943/mangiferaedu.v6i2.128.

Muharni, Muharni, Ferlinahayati, Fitrya, Eliza, Heni Yohandini, and Cindy Cenora. 2023. “Subchronic Toxicity Test of Sungkai Leaf Ethanol Extract (Paronema canescens Jack.) Against the White Rat Rattus noverticus (Wistar strain).” Jurnal Sains Farmasi & Klinis 10(2): 211-217. https://doi.org/10.25077/jsfk.10.2.211-217.2023.

Nabella, Visca, Dwi Susanti, and Ade Maria Ulfa. 2025. “Antibacterial Activity Test of Ethanol Extract of Arabian Bidara Leaf (Ziziphus spina-christi L.) Desf. Against Salmonella typhi and Bacillus cereus Bacteria.” CERATA Journal of Pharmaceutical Sciences 16(1): 60–70. https://doi.org/10.61902/cerata.v16i1.900.

Ngibad, Khoirul, Elis Dwi Nuryani, Tarisatus Syahlum Mukarromah, and Nurisma Fadhila. 2025. “Antihypercholesterolemic Potential of Ethanolic Leaf Extracts of Santalum album L., Calotropis gigantea, Ziziphus spina-christi L.” Jurnal Penelitian Pendidikan IPA 11(6): 846–852. https://doi.org/10.29303/jppipa.v11i6.11499.

Nofita, Nofita, Diah Ningrum Uli Rosidah, and Mashuri Yusuf. 2022. “Comparison of Antioxidant Activity of Bidara Leaf Extract (Ziziphus spina-christi L.) Using ethanol and n-hexane Solvents.” Journal of Medical and Health Sciences 9(3): 924-933. https://doi.org/10.33024/jikk.v9i3.5562.

Putri, Anike, Nofita Nofita, and Ade Maria Ulfa. 2023. “Comparison of Antioxidant Activity of Bidara Leaf Extract (Ziziphus spina-christi L.) with Percolation Extraction and Infusion Techniques.” Journal of Medical and Health Sciences 9(4): 1178–1189. https://doi.org/10.33024/jikk.v9i4.5635.

Rahmanto, Yuri. 2024. “Best Customer Selection Decision Support System Using SD-MOORA Method.” Journal of Artificial Intelligence and Technology Information (JAITI) 2(3): 163–172. https://doi.org/10.58602/jaiti.v2i3.135.

Sakalaty, Evanda Enggelina, Ratri Ariatmi Nugrahani, and Nurul Hidayati Fithriyah. 2024. “Antioxidant Inhibition Performance of Miana Leaf Extract (Coleus scutellarioides (L.) Benth.) at Extraction Time Variation and as an Additive to Liquid Bath Soap.” Chimica et Natura Acta 12(1): 1–9. https://doi.org/10.24198/cna.v12.n1.47348.

Sun, Meiling, Ying Wei, Xiaoguang Feng, Junfeng Fan, and Xiangning Chen. 2022. “Composition, Anti-LDL Oxidation, and Non-Enzymatic Glycosylation Inhibitory Activities of The Flavonoids from Mesembryanthemum crystallinum.” Frontiers in Nutrition 9: 1-17. https://doi.org/10.3389/fnut.2022.963858.

Ulfiah, Ayu, Arina F. Arifin, Rezky Pratiwi L., Sri Wahyuni Gayatri, and Nesyana Nurmadill. 2020. “Effectiveness of Moringa Leaf Extract on Blood Cholesterol Levels in Mice Trying Animals.” UMI Medical Journal 5(1): 28–37. https://doi.org/10.33096/umj.v5i1.86.

Wahyuni, Windi Tri, Fina Nikmatul Wasi’ah, Ismatun Maulidiyah, Elsa Saqila, Sirojul Eilma, Oktafia Damayanti, Brelianty Nevy, Silvi Novita, Agustinus Alfred Seran, Ivan Charles S.Klau, and Arista Wahyu Ningsih. 2023. Review “Article: Study of Phytochemistry and Pharmacological Activity on the Bidara Plant (Ziziphus mauritiana Lamk).” Scientific Journals and Student Papers 2(1): 53–62. https://doi.org/10.54066/jikma.v2i1.1287.

Wijaya, Heri, Siti Jubaidah, and Rukayyah. 2022. “Comparison of Extraction Methods on the Yield of Turi Stem Extract (Sesbania grandiflora L.) By Using the Maceration and Sokhletation Method.” Indonesian Journal of Pharmacy and Natural Product 5(1): 1–11. https://doi.org/10.35473/ijpnp.v5i1.1469.

Yuningrum, Hesti, Merita Eka Rahmuniyati, and Theresia Dwi Putri Lende. 2022. “Fried Food Consumption and Cholesterol Intake are related to the Incidence of Hypercholesterolemia in College Students.” Equatorial Health Journal (Public Health) 9(2): 98-108. https://doi.org/10.29406/jkmk.v9i2.3911.

Zhang, Yan, Xue-Ying Zhang, Shan-Rui Shi, Chao-Nan Ma, Yun-Peng Lin, Wen-Gang Song, and Shou-Dong Guo. 2024. “Natural Products In Atherosclerosis Therapy By Targeting PPARs: A Review Focusing On Lipid Metabolism And Inflammation.” Frontiers in Cardiovascular Medicine 11: 1372055. https://doi.org/10.3389/fcvm.2024.1372055.

DOI: http://dx.doi.org/10.52365/jecp.v6i1.1449