Uji Aktivitas Antibakteri Ekstrak Daun Pepaya (Carica papaya L.) terhadap Pertumbuhan Bakteri Vibrio cholerae dan Salmonella thypi

Nining Fadilah Utami, Dwi Rachmawaty, Arisanty Arisanty

Abstract


Tanaman  pepaya (Carica  papaya L.) sudah  lama dipakai untuk obat herbal serta dipercaya mempunyai kegunaan dalam pengobatan, salah satunya untuk memperlambat pertumbuhan pada bakteri. Adapun senyawa aktif yang ada di tumbuhan pepaya mempunyai kegunaan seperti karpain, alkaloid, flavonoid, saponin, dan tannin. Tujuan dari penelitian ini guna mengetahui aktivitas dari antibakteri pada ekstrak daun pepaya pada pertumbuhan Vibrio cholerae dan Salmonella thypi berdasarkan diameter zona hambat. Ekstrak dari daun pepaya diperoleh melalui metode maserasi lalu dilanjutkan dengan skrining fitokimia serta pengujian aktivitas antibakteri dengan memakai metode disc diffusion, kontrol positif digunakan kloramfenikol serta kontrol negatif digunakan DMSO. Hasil dari pengujian SPSS yaitu konsentrasi ekstrak 2% potensial dimanfaatkan untuk antibakteri guna Vibrio cholerae dan Salmonella thypi.

Papaya plants (Carica papaya L.) have long been used for herbal medicine and are believed to have medicinal uses, one of which is to slow the growth of bacteria. The active compounds in papaya plants have uses such as karpain, alkaloids, flavonoids, saponins, and tannins. The purpose of this study was to determine the antibacterial activity of papaya leaf extract on the growth of Vibrio cholerae and Salmonella thypi based on the diameter of the inhibition zone. Extract from papaya leaves was obtained through the maceration method, followed by phytochemical screening and antibacterial activity testing using the disc diffusion method. The positive control used chloramphenicol, and the negative control used DMSO. The results of the SPSS test showed that the 2% extract concentration could potentially be used as an antibacterial for Vibrio cholerae and Salmonella typhi.


Keywords


Aktivitas antibakteri; ekstrak daun pepaya; Salmonella thypi; Vibrio cholerae

Full Text:

PDF

References


Dziejman, M., Balon, E., Boyd, D., Fraser, C. M., Heidelberg, J. F., & Mekalanos, J. J. 2002. “Comparative Genomic Analysis of Vibrio cholerae: Genes that Correlate with Cholera Endemic and Pandemic Disease.” Proceedings of the National Academy of Sciences, 99 (3): https://doi.org/1556–1561. 10.1073/pnas.042667999

Herlina, I., Mandar, R. S. S., Puspawani, Y., & Meldawati, M. 2020. “Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) terhadap Pertumbuhan Bakteri Salmonella typhi.” (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat), 5(1). https://doi.org/10.37887/jimkesmas.v5i1.11105

Jawetz, M., Melnick, J. L., & Adelberg, E. A. 2005. “Mikrobiologi Kedokteran.” Jakarta: Penerbit Salemba Medika.

Mara, A. S., Gia, Susilo, J., & Viffta, R. L. 2020. “Perbedaan Pelarut Pengekstraksi Daun Afrika (Vernonia amygdalina Del.) terhadap Daya Hambat Pertumbuhan Pseudomonas aeruginosa.” Doctoral Dissertation, Universitas Ngudi Waluyo.

Matson, J. S., Withey, J. H., & DiRita, V. J. 2007. “Regulatory Networks Controlling Vibrio cholerae Virulence Gene Expression.” Infection and Immunity, 75(12), 5542–5549 https://doi.org/10.1128/iai.01094-07

Milind, P., & Gurditta, G. 2011. “Basketful Benefits of Papaya.” International Research Journal of Pharmacy, 2(7), 6–12.

Pendit, P. A. C. D., Zubaidah, E., & Sriherfyna, F. H. 2016. “Karakteristik Fisik-Kimia dan Aktivitas Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi L.).” Jurnal Pangan dan Agroindustri, 4(1).

Retnowati, Y., Bialangi, N., & Posangi, N. W. 2011. “Pertumbuhan Bakteri Staphylococcus aureus pada Media yang Diekspos dengan Infus Daun Sambiloto (Andrographis paniculata).” Jurnal Sainstek, 6(2).

Rini, A. A., & Supriatno, R. H. 2017. “Skrining Fitokimia dan Uji Antibakteri Ekstrak Etanol Buah Kawista (Limonia acidissima L.) dari Daerah Kabupaten Aceh Besar terhadap Bakteri Escherichia coli.” J Ilm Mahasis Fak Kegur Ilmu Pendid Unsyiah, 2(1), 1–12.

Tala, D. S., Gatsing, D., Fodouop, S. P. C., Fokunang, C., Kengni, F., & Djimeli, M. N. 2015. “In Vivo Anti-Salmonella Activity of Aqueous Extract of Euphorbia Prostrata Aiton (Euphorbiaceae) and its Toxicological Evaluation.” Asian Pacific Journal of Tropical Biomedicine, 5(4), 310. https://doi.org/10.1016/S2221-1691(15)30350-6

Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. 2011. “Phytochemical Screening and Extraction: a Review.” Internationale Pharmaceutica Sciencia, 1(1), 98–106.

Widiastuti, R., Mardiyaningsih, A., & Putri, Y. D. 2015. “Uji Aktivitas Ekstrak Etanol Daun Pepaya (Carica papaya) terhadap Waktu Kematian Cacing Ascaridia galli Schrank secara In Vitro.” Prosiding Seminar Nasional & Internasional.




DOI: http://dx.doi.org/10.52365/jecp.v3i2.678

Article Metrics

Abstract view : 492 times
PDF - 333 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Nining Fadilah Utami

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Indexed in:


  


Creative Commons License
Journal of Experimental and Clinical Pharmacy (JECP) by Department of Pharmacy, Poltekkes Kemenkes Gorontalo is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Support By: