Application of Natural Deep Eutectic Solvents (NADES) in the Natural Product of Antioxidant Activity

Andi Nurul Annisa Hidayah; Aktsar Roskiana Ahmad; Faradiba Abdul Rasyid

doi:10.52365/jecp.v5i2.1345

Application of Natural Deep Eutectic Solvents (NADES) in the Natural Product of Antioxidant Activity

Andi Nurul Annisa Hidayah, Aktsar Roskiana Ahmad, Faradiba Abdul Rasyid

Abstract

The extraction of bioactive compounds from plant-based materials poses significant challenges due to the limitations of traditional organic solvents, including toxicity and environmental impact. Natural Deep Eutectic Solvents (NADES), formed from natural components at defined molar ratios, have emerged as green, sustainable alternatives for efficient extraction. This literature review aims to investigate the applications, compositions, and effectiveness of NADES in extracting antioxidant-rich compounds from natural sources. Relevant peer-reviewed articles published within the last ten years were retrieved from Google Scholar and PubMed using keywords such as “NADES,” “natural product,” and “antioxidant activity.” Studies included using NADES to extract phytochemicals, pharmaceuticals, or food compounds and address their toxicity, safety, and physicochemical properties. Non-peer-reviewed articles, unrelated solvent systems, and duplicate studies were excluded. The review identified compelling NADES compositions, including choline chloride: lactic acid (1:2), choline chloride: oxalic acid (1:1), and citric acid: glycerol (1:1), which demonstrated high extraction efficiency and antioxidant activity. The tunability of hydrogen bond donor and acceptor ratios allows NADES to be optimized for specific bioactive compounds. In conclusion, NADES offers a promising and eco-friendly approach for future applications in natural product extraction.

Keywords

NADES; natural product; antioxidant activity

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References

Ahmad, Islamudin, Seliana Oklivia Prazitya Shakti, Wisnu Cahyo Prabowo, Baso Didik Hikmawan, M. Arifuddin, Marissa Angelina, Muhammad Eka Prastya, et al. 2024. “Citric Acid-Glycerol-Based NADES for Microwave-Assisted Extraction Enhances the Polyphenols Level of Eleutherine bulbosa Mill. Urb. Bulbs.” Journal of Applied Pharmaceutical Science 14 (9): 189–97. https://doi.org/10.7324/JAPS.2024.180291.

Alañón, M Elena, Milena Ivanović, Sandra Pimentel-Mora, Isabel Borrás-Linares, David Arráez-Román, and Antonio Segura-Carretero. 2020. “A Novel Sustainable Approach for the Extraction of Value-Added Compounds from Hibiscus sabdariffa L. Calyces by Natural Deep Eutectic Solvents.” Food Research International (Ottawa, Ont.) 137 (November): 109646. https://doi.org/10.1016/j.foodres.2020.109646.

Bosiljkov, Tomislav, Filip Dujmić, Marina Cvjetko Bubalo, Janez Hribar, Rajko Vidrih, Mladen Brnčić, Emil Zlatic, Ivana Radojčić Redovniković, and Stela Jokić. 2017. “Natural Deep Eutectic Solvents and Ultrasound-Assisted Extraction: Green Approaches for Extraction of Wine Lees Anthocyanins.” Food and Bioproducts Processing 102: 195–203. https://doi.org/10.1016/j.fbp.2016.12.005.

Bubalo, Marina Cvjetko, Senka Vidović, Ivana Radojčić Redovniković, and Stela Jokić. 2015. “Green Solvents for Green Technologies.” Journal of Chemical Technology and Biotechnology 90 (9): 1631–39. https://doi.org/10.1002/jctb.4668.

Chaves, Jaísa Oliveira, Mariana Corrêa de Souza, Laise Capelasso da Silva, Daniel Lachos-Perez, Paulo César Torres-Mayanga, Ana Paula da Fonseca Machado, Tânia Forster-Carneiro, et al. 2020. “Extraction of Flavonoids From Natural Sources Using Modern Techniques.” Frontiers in Chemistry 8 (September). https://doi.org/10.3389/fchem.2020.507887.

Dewi, Yuli Piana, Ida Zahrina, and Yelmida. 2021. “Karakteristik NADES (Natural Deep Eutectic Solvent).” Jom FTEKNIK 8 (1): 1–5. https://www.scribd.com/document/681150634/29468-57015-1-SM-2.

Doldolova, Khadija, Mustafa Bener, Melisa Lalikoğlu, Yavuz Selim Aşçı, Refik Arat, and Reşat Apak. 2021. “Optimization and Modeling of Microwave-Assisted Extraction of Curcumin and Antioxidant Compounds from Turmeric by Using Natural Deep Eutectic Solvents.” Food Chemistry 353 (August):129337. https://doi.org/10.1016/j.foodchem.2021.129337.

Faggian, Marta, Stefania Sut, Beatrice Perissutti, Valeria Baldan, Iztok Grabnar, and Stefano Dall’Acqua. 2016. “Natural Deep Eutectic Solvents (NADES) as a Tool for Bioavailability Improvement: Pharmacokinetics of Rutin Dissolved in Proline/Glycine after Oral Administration in Rats: Possible Application in Nutraceuticals.” Molecules 21 (11): 1–11. https://doi.org/10.3390/molecules21111531.

González-Laredo, Rubén F., Víctor Iván Sayago-Monreal, Martha Rocío Moreno-Jiménez, Nuria Elizabeth Rocha-Guzmán, José Alberto Gallegos-Infante, Luisa Fernanda Landeros-Macías, and Martha Rosales-Castro. 2023. “Natural Deep Eutectic Solvents (NaDES) as an Emerging Technology for the Valorisation of Natural Products and Agro-Food Residues: A Review.” International Journal of Food Science and Technology 58 (12): 6660–73. https://doi.org/10.1111/ijfs.16641.

Gu, Tongnian, Mingliang Zhang, Ting Tan, Jia Chen, Zhan Li, Qinghua Zhang, and Hongdeng Qiu. 2014. “Deep Eutectic Solvents as Novel Extraction Media for Phenolic Compounds from Model Oil.” Chem. Commun. 50 (79): 11749–52. https://doi.org/10.1039/C4CC04661G.

Guo, Na, Ping-Kou, Yi-Wei Jiang, Li-Tao Wang, Li-Jun Niu, Zhi-Ming Liu, and Yu-Jie Fu. 2019. “Natural Deep Eutectic Solvents Couple with Integrative Extraction Technique as an Effective Approach for Mulberry Anthocyanin Extraction.” Food Chemistry 296:78–85. https://doi.org/10.1016/j.foodchem.2019.05.196.

Huang, Yan, Jiehong Yang, Yu Zhao, Li Yu, Yu He, Haitong Wan, and Chang Li. 2021. “Screening, Optimization, and Bioavailability Research of Natural Deep Eutectic Solvent Extracts from Radix Pueraria.” Molecules 26 (3): 1–11. https://doi.org/10.3390/molecules26030729.

Ivanović, Milena, Alen Albreht, Peter Krajnc, Irena Vovk, and Maša Islamčević Razboršek. 2021. “Sustainable Ultrasound-Assisted Extraction of Valuable Phenolics from Inflorescences of Helichrysum arenarium L. using Natural Deep Eutectic Solvents.” Industrial Crops and Products 160: 113102. https://doi.org/10.1016/j.indcrop.2020.113102.

Latifah, Fadzil, and Muhammad Nuh. 2024. “Pengaruh Metode Microwave-Assisted Extraction (MAE) dengan Pelarut Natural Deep Eutectic Solvent (NADES) Ekstrak Daun Mangga Gedong Terhadap Kadar Total Flavonoid dan Aktivitas Antioksidan.” Jurnal Ilmiah Ibnu Sina 9 (1): 89–98. https://doi.org/10.36387/jiis.v9i1.1628.

López-Salazar, Herminia, Brenda Hildeliza Camacho-Díaz, Martha Lucia Arenas Ocampo, and Antonio Ruperto Jiménez-Aparicio. 2023. “Microwave-Assisted Extraction of Functional Compounds from Plants: A Review.” Bioresources 18 (3): 6614–38. https://doi.org/10.15376/biores.18.3.Lopez-Salazar.

Mansinhos, Inês, Sandra Gonçalves, Raquel Rodríguez-Solana, José Luis Ordóñez-Díaz, José Manuel Moreno-Rojas, and Anabela Romano. 2021. “Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata Subsp. Lusitanica (Chaytor) Franco.” Antioxidants (Basel, Switzerland) 10 (4). https://doi.org/10.3390/antiox10040582.

Nurbaeti, Adinda Elsam. 2023. “Uji Aktivitas Antioksidan Ekstrak Jahe Merah dengan Pelarut Nades Kombinasi Betain : Asam.” Universitas Pakuan.

Panjaitan, Franz Eliezer. 2024. “Uji Aktivitas Antioksidan, Penetapan Kadar Fenolik dan Flavonoid Total Serta Identifikasi Kandungan Kimia Ekstrak Propolis NADES.” Universitas Pancasila.

Popovic, Boris M, Nikola Micic, Aleksandar Potkonjak, Bojana Blagojevic, Ksenija Pavlovic, Dubravka Milanov, and Tatjana Juric. 2022. “Novel Extraction of Polyphenols from Sour Cherry Pomace Using Natural Deep Eutectic Solvents - Ultrafast Microwave-Assisted NADES Preparation and Extraction.” Food Chemistry 366 (January): 130562. https://doi.org/10.1016/j.foodchem.2021.130562.

Radošević, Kristina, Iva Čanak, Manuela Panić, Ksenija Markov, Marina Cvjetko Bubalo, Jadranka Frece, Višnja Gaurina Srček, and Ivana Radojčić Redovniković. 2018. “Antimicrobial, Cytotoxic and Antioxidative Evaluation of Natural Deep Eutectic Solvents.” Environmental Science and Pollution Research 25 (14): 14188–96. https://doi.org/10.1007/s11356-018-1669-z.

Satija, Pinky, Anamika Chambyal, Gurleen Singh, Ritvik, Gurjaspreet Singh, Swati Devi, and Jandeep Singh. 2024. “Natural Deep Eutectic Solvents (NADES): Manufacture, Characteristics, and Their Significance as Designer Solvents.” Chemistry Select 9 (22): e202401212. https://doi.org/10.1002/slct.202401212.

Sebbah, Tarik, Imene Yahla, Edite Cunha, Ali Riazi, Célia G. Amorim, Joan Manuel Rodriguez-Diaz, and Maria C.B.S.M. Montenegro. 2025. “Enhanced Extraction and Separation with HPLC-DAD of Phenolic and Flavonoid Antioxidants from Portulaca oleracea L. Leaves Using Tailored Terpenoid-Based NADES: Comparative Assessment of Antiradical and Antimicrobial Activities.” Antioxidants 14 (2). https://doi.org/10.3390/antiox14020132.

Shikov, Alexander N., Vera M. Kosman, Elena V. Flissyuk, Irina E. Smekhova, Abdelhameed Elameen, and Olga N. Pozharitskaya. 2020. “Natural Deep Eutectic Solvents for the Extraction of Phenyletanes and Phenylpropanoids of Rhodiola rosea L.” Molecules 25 (8): 9–11. https://doi.org/10.3390/molecules25081826.

Silva, Dariane Trivisiol da, Franciele Aline Smaniotto, Isabella Ferreira Costa, Julia Baranzelli, Aline Muller, Sabrina Somacal, Camila Sant’Anna Monteiro, et al. 2021. “Natural Deep Eutectic Solvent (NADES): A Strategy to Improve the Bioavailability of Blueberry Phenolic Compounds in a Ready-to-Use Extract.” Food Chemistry 364 (December):130370. https://doi.org/10.1016/j.foodchem.2021.130370.

Smith, Emma L., Andrew P. Abbott, and Karl S. Ryder. 2014. “Deep Eutectic Solvents (DESs) and Their Applications.” Chemical Reviews 114 (21): 11060–82. https://doi.org/10.1021/cr300162p.

Villa, Carla, Debora Caviglia, Francesco Saverio Robustelli della Cuna, Guendalina Zuccari, and Eleonora Russo. 2024. “NaDES Application in Cosmetic and Pharmaceutical Fields: An Overview.” Gels 10 (2): 1–18. https://doi.org/10.3390/gels10020107.

Yang, Zhao, Shi-Jun Yue, Huan Gao, Qiao Zhang, Ding-Qiao Xu, Jing Zhou, Jia-Jia Li, and Yu-Ping Tang. 2023. “Natural Deep Eutectic Solvent-Ultrasound Assisted Extraction: A Green Approach for Ellagic Acid Extraction from Geum japonicum.” Frontiers in Nutrition 9 (9): 1079767. https://doi.org/10.3389/fnut.2022.1079767.

Yulianita, Zaldy Rusli, Usep Suhendar, and Zulfa Masrani. 2022. “Ekstraksi Flavonoid Daun Meniran Menggunakan Pelarut Natural Deep Eutectic Solvent Berbasis Kolin Klorida-Asam dengan Ultrasound Assisted Extraction.” FITOFARMAKA: Jurnal Ilmiah Farmasi 12 (1): 46–57. https://doi.org/10.33751/jf.v12i1.4294.

DOI: http://dx.doi.org/10.52365/jecp.v5i2.1345