Potensi Ekstrak Etanol Daun Murbei (Morus alba L.) sebagai Antifungi terhadap Jamur Candida albicans
Abstract
Senyawa kimia di daun murbei (Morus alba L.), termasuk flavonoid, tanin, steroid, dan saponin. Salah satu golongan senyawa yaitu flavonoid memiliki sifat antioksidan, antibakteri, dan sifat antimikroba yang dapat meningkatkan kontraksi luka. Penelitian dilakukan untuk mengetahui bagaimana potensi daun murbei sebagai antifungi terhadap jamur Candida albicans. Untuk menghasilkan ekstrak yang akan diskrining fitokimia, teknik maserasi menggunakan pelarut etanol 70% digunakan untuk ekstraksi sampel. Uji bebas etanol dan dilanjutkan dengan pengujian antifungi terhadap jamur Candida albicans. Hasil ekstraksi memperoleh persen rendemen ekstrak 3,89%. Pengujian skrining fitokimia mengandung flavonoid, saponin dan tannin. Pengujian ekstrak dengan konsentrasi 1,25%, 2,5%, 5% dan 10% memiliki aktivitas sebagai antifungi terhadap Candida albicans yaitu 9,7 mm ± 0,36, 10,83 mm ± 0,45, 15,8 mm ± 3,31, 26,06 mm ± 3,27 dan ketokonazole sebagai kontrol positif 33,4 mm ± 0,57. Disimpulkan bahwa ekstrak etanol daun murbei memiliki aktivitas sebagai antifungi terhadap Candida albicans. Ekstrak etanol daun murbei dapat menghambat pertumbuhan Candida albicans hingga konsentrasi 1,25%, sehingga dapat dimanfaatkan sebagai antijamur.
Chemical compounds in mulberry leaves (Morus alba L.) include flavonoids, tannins, steroids, and saponins. One class of compounds, namely flavonoids, has antioxidant, antibacterial, and antimicrobial properties that can increase wound contraction. Research was conducted to determine the potential of mulberry leaves as an antifungal against the fungus Candida albicans. To produce extracts that will be screened for phytochemicals, a maceration technique using 70% ethanol solvent is used for sample extraction. Ethanol-free test and continued with antifungal testing against Candida albicans fungus. The extraction results obtained an extract yield percentage of 3.89%. Phytochemical screening tests contain flavonoids, saponins, and tannins. Extract testing with concentrations of 1.25%, 2.5%, 5%, and 10% had antifungal activity against Candida albicans, namely 9.7 mm ± 0.36, 10.83 mm ± 0.45, 15.8 mm ± 3.31, 26.06 mm ± 3.27, and ketoconazole as a positive control (33.4 mm ± 0.57). It was concluded that the ethanol extract of mulberry leaves has antifungal activity against Candida albicans. Mulberry leaf ethanol extract can inhibit the growth of Candida albicans up to a concentration of 1.25%, so it can be used as an antifungal.
Keywords
Full Text:
PDFReferences
Aboody, Mohammed Saleh Al, and Suresh Mickymaray. 2020. “Anti-Fungal Efficacy and Mechanisms of Flavonoids.” Antibiotics 9 (2). https://doi.org/10.3390/antibiotics9020045.
Alioes, Yustini, and Amalia Kartika. 2019. “Uji Potensi Antijamur Candida albicans Ekstrak Daun Gelinggang (Cassia alata L.) dibandingkan dengan Sediaan Daun Sirih yang Beredar di Pasaran Secara in Vitro.” Jurnal Kimia Riset 3 (2): 108. https://doi.org/10.20473/jkr.v3i2.12040.
Arundany, Achwana Sri. 2011. “Uji Aktivitas Ekstrak Etanol Daun Ketepeng Cina sebagai Antiskabies Secara In Vitro.” Universitas Jember. https://repository.unej.ac.id/bitstream/handle/123456789/23772/K %2875%29k_1kedokteran.pdf?sequence=1&isAllowed=y.
Dalimartha, Setiawan. 2006. Atlas Tumbuhan Obat Jilid 4. Jakarta: Puspa Swara.
Fitriani, Endang Wahyu, Erlina Imelda, Christina Kornelis, and Christina Avanti. 2016. “Karakterisasi dan Stabilitas Fisik Mikroemulsi Tipe A/M dengan Berbagai Fase Minyak.” Pharmaceutical Sciences and Research 3 (1): 31–44. https://doi.org/10.7454/psr.v3i1.3221.
Hastuti, Utami Sri, Anggia Oktantia, and N Nurhamidah. 2012. “Daya Antibakteri Ekstrak Daun dan Buah Murbei (Morus alba L.) Terhadap Staphylococcus aureus dan Shigella dysenteriae.” Seminar Nasional IX Pendidikan Biologi 9: 530–34. https://jurnal.fkip.uns.ac.id/index.php/prosbio/article/view/1142.
Hersila, Natasya, Moralitha Chatri, Vauzia, and Irdawati. 2023. “Senyawa Metabolit Sekunder (Tanin) pada Tanaman sebagai Antifungi.” Jurnal Embrio 15 (1): 16–22. https://doi.org/1031317/embrio
Imrawati, Imrawati, Yuri Pratiwi Utami, and Hardianto Hardianto. 2023. “Antioxidant Activity of Leilem Leaf Extract Fractions Clerodendrum minahassae Teijsm. and Binn. using the DPPH Method (2,2-Diphenyl-1-Picrylhydrazil).” International Journal of Health Sciences (IJHS) 1 (4): 911–23. https://doi.org/10.59585/ijhs.v1i4.220
Imrawati, Imrawati, Yuri Pratiwi Utami, and Ade Ainun Insani. 2023. “Identification of Compound Content and Antioxidant Activity Test of Ethanol Extract of Stachytarpheta jamaicensis Vahl. Leaf ABTS Method.” International Journal of Research in Pharmacy and Pharmaceutical Sciences 8 (3): 10–15.
Lenggu, Claritha Kaci Louis, Desi Indria Rini, and Anita Lidesna Shinta. 2020. “Uji Aktivitas Ekstrak Etanol Kulit Daging Buah Lontar (Borassus flabellifer Linn) Terhadap Pertumbuhan Escherichia coli Secara in Vitro.” Cendana Medical Journal (CMJ) 8(2) (April): 96–107. https://doi.org/10.35508/cmj.v8i2.3353
Pribadi, Hamdanah, and Eko Sugeng. 2012. “Keragaman Kepekaan Candida albicans yang Diisolasi dari Lokasi Peternakan Sapi Perah terhadap Beberapa Anticendawan.” Institut Pertanian Bogor.
Setiawan, Eko Nur, Nur Mita, and Arsyik Ibrahim. 2015. “Karakterisasi dan Identifikasi Metabolit Sekunder Isolat Jamur Endofit Daun Sukun (Artocarpus altilis).” Prosiding Seminar Nasional Kefarmasian Ke-2 2: 24–25.
Silvina, S. 2006. “Uji Banding Efektivitas Ekstrak Rimpang Lengkuas (Alpinia galanga) 10% dengan Ketokonazol 2% Secara In Vitro Terhadap Pertumbuhan Candida albicans Pada Kandidiasis Vaginalis.” Universitas Diponegoro. http://eprints.undip.ac.id/21304/1/Silvina.pdf.
Simanjuntak, Helen Anjelina, and Megawati Butar-butar. 2019. “Uji Aktivittas Antifungi Ekstrak Etanol Umbi Bawang Merah.” Jurnal Penelitian dan Pembelajaran MIPA 4: 91–98. http://dx.doi.org/10.31604/eksakta.v4i2.91-98
Tee, Vincet, and Sugiarto Puradisastra. 2007. “Pengaruh Ekstrak Etanol Daun Murbei (Morus alba L.) terhadap Durasi Penyembuhan Luka Insisi pada Mencit Swiss Webster.” Fakultas Kedokteran Universitas Kristen Maranatha, No. 17.
Tiwari, Prashant, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, and Harleen Kaur. 2011. “Phytochemical Screening and Extraction: A Review.” Internationale Pharmaceutica Sciencia 1 (1): 98–106. https://doi.org/10.1002/hep.29375.
Utami, Y., P. Ismail, I. Nisa, and I. Oktaviani. 2023. “Antibacterial Activity Test of Etlingera elatior (Jack) R.M. Sm Leaf Extract against Bacteria Staphylococcus aureus and Escherichia coli Diffusion Method.” International Journal of Research in Pharmacy and Pharmaceutical Sciences 8 (3): 1–4.
Utami, Yuri Pratiwi, and Ainun Jariah. 2023. “Skrining Fitokimia dan Uji Sitotoksik Ekstrak Etanol Daun Andong Merah (Cordyline fruticosa).” Jurnal Katalisator 8 (1): 156–65. http://doi.org/10.22216/jk.v5i2.5717http://ejournal.kopertis10.or.id/index.php/katalisator.
Utami, Yuri Pratiwi, Risfah Yulianty, Yulia Yusrini Djabir, Gemini Alam, Sulawesi Selatan, Departemen Farmasi, Fakultas Farmasi, Universitas Hasanuddin, Fakultas Ilmu Kesehatan, and Universitas Almarisah Madani. 2023. “Antioksidan Ekstrak Daun Patikala (Etlingera elatior (Jack) R.M. Smith) Penangkal Radikal Bebas ABTS Asal Enrekang Sulawesi Selatan.” Jurnal Katalisator 8 (2): 291–300. http://doi.org/10.22216/jk.v5i2.5717http://ejournal.kopertis10.or.id/index.php/katalisator.
Widyaningrum, Herlina. 2019. Kitab Tanaman Obat Nusantara: Disertai Indeks Pengobatan. Jakarta: Media Pressindo.
Yulia, Resti, Moralita Chatri, Linda Advinda, and Dezi Handayani. 2023. “Saponins Compounds as Antifungal Against Plant Pathogens.” Serambi Biologi 8 (2): 2023.
Zainal, Tuti Handayani, Michrun Nisa, Saldi Hapiwaty, and Alfrita Sarrin. 2016. “Formulasi Emulgel Ekstrak Etanol Daun Senggani (Melastoma malabathricum L.) sebagai Luka Bakar.” Jurnal Inovasi Penelitian 3 (2): 1–23. https://doi.org/10.47492/jip.v3i2.1710
DOI: http://dx.doi.org/10.52365/jecp.v4i1.969
Article Metrics


Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Yuri Pratiwi Utami

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indexed in:
![]() Journal of Experimental and Clinical Pharmacy (JECP) by Department of Pharmacy, Poltekkes Kemenkes Gorontalo is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. | Support By: |