In Silico Exploration of Suruhan Leaves (Peperomia pellucida) for Triple-Negative Breast Cancer Therapy Targeting AURKA

Anwar Rovik; Vania Uly Andyra; Laelatul Afifah

doi:10.52365/jecp.v4i2.1119

In Silico Exploration of Suruhan Leaves (Peperomia pellucida) for Triple-Negative Breast Cancer Therapy Targeting AURKA

Anwar Rovik, Vania Uly Andyra, Laelatul Afifah

Abstract

Breast cancer is the most common type of cancer worldwide. One of the most widely used chemotherapeutic agents in the treatment of breast cancer is doxorubicin. However, doxorubicin has been reported to cause side effects. Suruhan (Peperomia pellucida) contains bioactive compounds that might protect against breast cancer cells. This study aims to analyze the bioactive compound activity of Suruhan leaves in triple-negative breast cancer in silico. The study was conducted by reviewing published literature on bioactive compounds in Suruhan leaves, predicting the protein target, analyzing the gene expression in triple-negative breast cancer (TNBC) patients, analyzing the protein interactions, and examining patient survival. There are five common bioactive compounds in Suruhan leaves, including peperomin A; 6,8-dihydroxy kaempferol 3,6,7,4'-tetramethyl ether 8-neohesperidoside; dillapiole; carotol; and pellucidin A. AURKA is a target protein for bioactive compounds that are also overexpressed in TNBC patients. AURKA has strong interactions with many proteins, including TPX2, NEDD9, CCNB2, CDC20, PLK1, BIRC5, INCENP, TACC3, TP53, and MYCN. The AURKA has emerged as a promising target for TNBC therapy.

Keywords

Drug discovery; ethnopharmacology; Peperomia pellucida; triple-negative breast cancer

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References

Al-Madhagi, Wafa M., Najihah Mohd Hashim, Nasser A.Awad Ali, Abeer A. Alhadi, Siti Nadiah Abdul Halim, and Rozana Othman. 2018. “Chemical Profiling and Biological Activity of Peperomia blanda (Jacq.) Kunth.” PeerJ 2018 (6): 1–19. https://doi.org/10.7717/peerj.4839.

Angelina, Marissa, Marya Salfia Khoerunisah, Kasiyati, Alfian Mahardika Forentin, and Muhammad Anwar Djaelani. 2024. “Synergistic Cytotoxicity Effect by Combination of N-Hexane Fraction of the Herbs (Peperomia pellucida) with Doxorubicin against Breast Cancer Cells (MCF-7).” South African Journal of Botany 170: 260–70. https://doi.org/10.1016/j.sajb.2024.03.049.

Clemen-Pascual, Lydia M, Rene Angelo S Macahig, and Nina Rosario L Rojas. 2021. “Comparative Toxicity, Phytochemistry, and Use of 53 Philippine Medicinal Plants.” Toxicology Reports 10 (9): 22–35. https://doi.org/10.1016/j.toxrep.2021.12.002.

Du, Ruijuan, Chuntian Huang, Kangdong Liu, Xiang Li, and Zigang Dong. 2021. “Targeting AURKA in Cancer: Molecular Mechanisms and Opportunities for Cancer Therapy.” Molecular Cancer 20 (1): 1–27. https://doi.org/10.1186/s12943-020-01305-3.

François, Tchoumbougnang, Jazet Dongmo Pierre Michel, Wouatsa Nangué Arlette Vyry, Fekam Boyom Fabrice, Sameza Modeste Lambert, Amvam Zollo Paul Henri, and Menut Chanta. 2013. “Composition and Antifungal Properties of Essential Oils from Five Plants Growing in the Mountainous Area of the West Cameroon.” Journal of Essential Oil Bearing Plants 16 (5): 679–88. https://doi.org/10.1080/0972060X.2013.764205.

Gomes, Paulo Wender P., Hugo Barretto, José Diogo E. Reis, Abraão Muribeca, Alice Veloso, Carlos Albuquerque, Andrew Teixeira, et al. 2022. “Chemical Composition of Leaves, Stem, and Roots of Peperomia pellucida (L.) Kunth.” Molecules 27 (6): 1–12. https://doi.org/10.3390/molecules27061847.

Kementerian Kesehatan RI. 2023. “Kanker Payudara Paling Banyak Di Indonesia.” 2023. https://sehatnegeriku.kemkes.go.id/.

Kurniawan, Agus, Fadlina Chany Saputri, Rissyelly, Islamudin Ahmad, and Abdul Mun’im. 2016. “Isolation of Angiotensin Converting Enzyme (ACE) Inhibitory Activity Quercetin from Peperomia pellucida.” International Journal of PharmTech Research 9 (7): 115–21.

Lüönd, Fabiana, Nami Sugiyama, Ruben Bill, Laura Bornes, Carolina Hager, Fengyuan Tang, Natascha Santacroce Christian Beisel, et al. 2021. “Distinct Contributions of Partial and Full EMT To Breast Cancer Malignancy.” Developmental Cell 56 (23): 3203–21. https://doi.org/10.1016/j.devcel.2021.11.006.

Mahmoud, Rinad, Paloma Ordóñez-Morán, and Cinzia Allegrucci. 2022. “Challenges for Triple Negative Breast Cancer Treatment: Defeating Heterogeneity and Cancer Stemness.” Cancers 14 (17): 1–15. https://doi.org/10.3390/cancers14174280.

Majrashi, Taghreed A., Saad Ali Alshehri, Abdulrhman Alsayari, Abdullatif Bin Muhsinah, Mohammad Alrouji, Asma M. Alshahrani, Anas Shamsi, and Akhtar Atiya. 2023. “Insight into the Biological Roles and Mechanisms of Phytochemicals in Different Types of Cancer: Targeting Cancer Therapeutics.” Nutrients 15 (7): 1-27. https://doi.org/10.3390/nu15071704.

Moorthi, Narayana, K Vasantha, and A Maruthasalam. 2018. “In Vitro Evaluation of Cytotoxic Properties of Peperomia pellucida ( L .).” Bioscience Discovery 9 (3): 344–55.

Ng, Bryant, Herindita Puspitaningtyas, Juan Adrian Wiranata, Susanna Hilda Hutajulu, Irianiwati Widodo, Nungki Anggorowati, Guardian Yoki Sanjaya, Lutfan Lazuardi, and Patumrat Sripan. 2023. “Breast Cancer Incidence in Yogyakarta, Indonesia from 2008–2019: A Cross-Sectional Study Using Trend Analysis and Geographical Information System.” PLoS ONE 18 (7 JULY): 1–17. https://doi.org/10.1371/journal.pone.0288073.

Nounou, Mohamed I., Fatema Elamrawy, Nada Ahmed, Kamilia Abdelraouf, Satyanarayana Goda, and Hussaini Syed-Sha-Qhattal. 2015. “Breast Cancer: Conventional Diagnosis and Treatment Modalities and Recent Patents and Technologies Supplementary Issue: Targeted Therapies in Breast Cancer Treatment.” Breast Cancer: Basic and Clinical Research 9: 17–34. https://doi.org/10.4137/BCBCR.S29420.

Oliveira, José C.S. De, Claudio A.G. Da Camara, Roberta C.S. Neves, and Priscilla S. Botelho. 2017. “Chemical Composition and Acaricidal Activity of Essential Oils from Peperomia pellucida Kunth. against Tetranychus urticae.” Revista Virtual de Quimica 9 (6): 2204–13. https://doi.org/10.21577/1984-6835.20170130.

Purwanti, Susi, Nursari Abdul Syukur, and Cristinawati B.R. Haloho. 2021. “Faktor Risiko Berhubungan dengan Kejadian Kanker Payudara Wanita.” Jurnal Bidan Cerdas 3 (4): 168–75. https://doi.org/10.33860/jbc.v3i4.460.

Rovik, Anwar, Vania Uly Andyra, Laelatul Afifah, and Nur Rokhmalia. 2024. “Etnobotani dan Potensi Suruhan (Peperomia pellucida) sebagai Bahan Obat Herbal.” Journal of Biomedical Science and Health 2 (2): 53–66. https://doi.org/10.4137/BCBCR.S29420.

Ruslan, Nur Batrisyia, Indah Mohd Amin, Narimah Abdul Hamid Hasani, Vivi Noryati Ahmad, Farizan Aris, and Khor Goot Heah. 2021. “In Vitro Cytotoxic Evaluation and Apoptotic Effects of Dillapiole on Human Nasal Squamous Cell Carcinoma.” Jurnal Teknologi 83 (5): 93–99. https://doi.org/10.11113/jurnalteknologi.v83.17077.

Siggelkow, Wulf, Daniel Boehm, Susanne Gebhard, Marco Battista, Isabel Sicking, Antje Lebrecht, Christine Solbach, et al. 2012. “Expression of Aurora Kinase a is Associated with Metastasis-Free Survival in Node-Negative Breast Cancer Patients.” BMC Cancer 12 (May 2014). https://doi.org/10.1186/1471-2407-12-562.

Su Xu, Na Li, Meng-Meng Ning, Cai-Hong Zhou, Qiao-Rong Yang, and Ming-Wei Wang. 2005. “Bioactive Compounds from Peperomia pellicuda.” American Chemical Society and America Society of Pharmacognosy, 247–50. https://doi.org/10.1021/np050457s.

Sun, Zhaoran, Dongmei Zhou, Jinkui Yang, and Daoyong Zhang. 2022. “Doxorubicin Promotes Breast Cancer Cell Migration and Invasion via DCAF13.” FEBS Open Bio 12 (1): 221–30. https://doi.org/10.1002/2211-5463.13330.

Verma, Ram S., Rajendra C. Padalia, Prakash Goswami, and Amit Chauhan. 2015. “Essential Oil Composition of Peperomia pellucida (L.) Kunth from India.” Journal of Essential Oil Research 27 (2): 89–95. https://doi.org/10.1080/10412905.2014.982878.

Winter, Pawel, Malgorzata Fuksiewicz, Agnieszka Jagiello-Gruszfeld, Zbigniew Nowecki, and Beata Kotowicz. 2023. “Expression of Soluble form of Aurora A as a Predictive Factor for Neoadjuvant Therapy in Breast Cancer Patients: a Single-Center Pilot Study.” Cancers 15 (22). https://doi.org/10.3390/cancers15225446.

World Health Organization. 2023. “Breast Cancer.” 2023. https://www.who.int/news-room/fact-sheets/detail/breast-cancer.

DOI: http://dx.doi.org/10.52365/jecp.v4i2.1119